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After pre-blocking, pancreas samples were stained with primary antibodies in 5% v/v goat serum

The detailed diagrams, the passionate arguments for subcutaneous injections right next to the patellar tendon, and the equally passionate counterarguments that it makes absolutely no difference

To test this hypothesis we performed a classic twin study

Mitochondrial respiration ( JO 2 ) 2 Tissues or cells were homogenized in MHSE buffer (70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1 mM EGTA, pH 7.2) plus 0.5 % (w/v) fatty-acid free BSA using a motorized Teflon glass homogenizer